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American Journal of Innovative Research & Applied Sciences
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| | ARTICLES | Am. J. innov. res. appl. sci. Volume 7, Issue 3, Pages 186-198 (September 2018) |
| Research Article |
American Journal of innovative
Research & Applied Sciences
Research & Applied Sciences
ISSN 2429-5396 (Online)
OCLC Number: 920041286
OCLC Number: 920041286
| SEPTEMBER VOLUME 7 | N° 3 | 2018 |
Authors Contact
*Correspondant author and authors Copyright © 2018:
| Siva Kamalakannan 1*| Marimuthu Ramar 1 | Ponnan Arumugam 1 | Kalimuthu Kovendan 2 | Balamurugan Chandramohan 1 | Douglas Vieira Thomaz 3 | Panagal Mani 4 | Selvaraju Raja 5 | and | Kadarkarai Murugan 1|
*Correspondant author and authors Copyright © 2018:
| Siva Kamalakannan 1*| Marimuthu Ramar 1 | Ponnan Arumugam 1 | Kalimuthu Kovendan 2 | Balamurugan Chandramohan 1 | Douglas Vieira Thomaz 3 | Panagal Mani 4 | Selvaraju Raja 5 | and | Kadarkarai Murugan 1|
Affiliation.
1. 1Division of Medical Entomology | Department of Zoology | Bharathiar University | Coimbatore-641 046, Tamil Nadu | India |
2. Division of Vector Biology and Control | Department of Zoology | Annamalai University | Annamalai Nagar, 608 002 | India |
3. Department of Pharmacy | Federal University of Goiás, Goiânia - 74605-170, Goiás, Brazil |
4. Department of Biotechnology, Annai College of Arts and Science | Kumbakonam |Tamil Nadu | India |
5.PG & Research Dept. of Zoology | Kongunadu Arts and Science College | GN Mills, Coimbatore- 641029, Tamil Nadu | India |
1. 1Division of Medical Entomology | Department of Zoology | Bharathiar University | Coimbatore-641 046, Tamil Nadu | India |
2. Division of Vector Biology and Control | Department of Zoology | Annamalai University | Annamalai Nagar, 608 002 | India |
3. Department of Pharmacy | Federal University of Goiás, Goiânia - 74605-170, Goiás, Brazil |
4. Department of Biotechnology, Annai College of Arts and Science | Kumbakonam |Tamil Nadu | India |
5.PG & Research Dept. of Zoology | Kongunadu Arts and Science College | GN Mills, Coimbatore- 641029, Tamil Nadu | India |
This article is made freely available as part of this journal's Open Access: ID | Siva-ManuscriptRef.3-ajira010718 |
|
ABSTRACT
Background: Biofabrication of silver nanoparticles from plant origin provides more resistance against mosquitoes. In the present study, silver nanoparticles (AgNPs) synthesized from the novel plant, Menyanthes trifoliata for the control of chikungunya vector, Aedes aegypti. Objective: The nanoparticles were evaluated for their toxicity of larvae, pupae and insect growth regulatory activity, persistent study against Aedes aegypti. Methods: Nanoparticles were analyzed with UV-vis spectrophotometry, SEM, XRD, FTIR, and EDAX. The characterization studies confirmed that AgNPs are of size 10-50nm and spherical in shape. The efficacy of plant-synthesized AgNPs was tested against different concentrations ranging from 5-25 µg/mL against L1 to L4 larval instars and pupae of A. aegypti. Results: Observed value of LC50 = 8.17, 9.78, 12.96, 16.73 and 20.11 µg/mL; the LC90 = 19.57, 24.25, 29.64, 36.95 and 41.81 µg/mL for treated all larval instars and pupae, respectively. The mortality rates were positively correlated with the concentration of AgNPs. Significant (P<0.05) changes in larval mortality were also recorded between the period of exposure to all larval instars of A. aegypti. The larval and pupal growth was significantly diminished (IGR activity) by the action of AgNPs and its emergence inhibition EI50 and EI90 values are 1.87 (10.58) significantly noted against A. aegypti. Conclusion: The study reported that the toxic nature of AgNPs had a significantly higher impact on the third instars, followed by other stages, and their effect lasted up to a 12-week period. Larval midgut layer strongly disrupted and it’s showed in the histology section. These findings reported M. trifoliata synthesized AgNPs are rapid, eco-friendly, and novel mosquito control agents.
Keywords: Menyanthes trifoliate, Silver Nanoparticles, Mortality, Insect growth regulatory, Histology
Background: Biofabrication of silver nanoparticles from plant origin provides more resistance against mosquitoes. In the present study, silver nanoparticles (AgNPs) synthesized from the novel plant, Menyanthes trifoliata for the control of chikungunya vector, Aedes aegypti. Objective: The nanoparticles were evaluated for their toxicity of larvae, pupae and insect growth regulatory activity, persistent study against Aedes aegypti. Methods: Nanoparticles were analyzed with UV-vis spectrophotometry, SEM, XRD, FTIR, and EDAX. The characterization studies confirmed that AgNPs are of size 10-50nm and spherical in shape. The efficacy of plant-synthesized AgNPs was tested against different concentrations ranging from 5-25 µg/mL against L1 to L4 larval instars and pupae of A. aegypti. Results: Observed value of LC50 = 8.17, 9.78, 12.96, 16.73 and 20.11 µg/mL; the LC90 = 19.57, 24.25, 29.64, 36.95 and 41.81 µg/mL for treated all larval instars and pupae, respectively. The mortality rates were positively correlated with the concentration of AgNPs. Significant (P<0.05) changes in larval mortality were also recorded between the period of exposure to all larval instars of A. aegypti. The larval and pupal growth was significantly diminished (IGR activity) by the action of AgNPs and its emergence inhibition EI50 and EI90 values are 1.87 (10.58) significantly noted against A. aegypti. Conclusion: The study reported that the toxic nature of AgNPs had a significantly higher impact on the third instars, followed by other stages, and their effect lasted up to a 12-week period. Larval midgut layer strongly disrupted and it’s showed in the histology section. These findings reported M. trifoliata synthesized AgNPs are rapid, eco-friendly, and novel mosquito control agents.
Keywords: Menyanthes trifoliate, Silver Nanoparticles, Mortality, Insect growth regulatory, Histology
EXPLORATION OF DISTINCTIVE MENYANTHES TRIFOLIATA AS GENERATED GREEN NANOPARTICLES: REPORT THEIR LETHAL TOXICITY AGAINST Aedes Aegypti
| Siva Kamalakannan 1*| Marimuthu Ramar 1 | Ponnan Arumugam 1 | Kalimuthu Kovendan 2 | Balamurugan Chandramohan 1 | Douglas Vieira Thomaz 3 | Panagal Mani 4 | Selvaraju Raja 5 | and | Kadarkarai Murugan 1 |. Am. J. innov. res. appl. sci. 2018; 7(3):186-198.
| PDF FULL TEXT | | XML FILE | | Received | 01 September 2018 | | Accepted| 24 September 2018 | | Published | 28 September 2018 |
| Siva Kamalakannan 1*| Marimuthu Ramar 1 | Ponnan Arumugam 1 | Kalimuthu Kovendan 2 | Balamurugan Chandramohan 1 | Douglas Vieira Thomaz 3 | Panagal Mani 4 | Selvaraju Raja 5 | and | Kadarkarai Murugan 1 |. Am. J. innov. res. appl. sci. 2018; 7(3):186-198.
| PDF FULL TEXT | | XML FILE | | Received | 01 September 2018 | | Accepted| 24 September 2018 | | Published | 28 September 2018 |