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  | ARTICLE  | Am. J. innov. res. appl. sci. Volume 11,  Issue 6, Pages 165-174 (December 2020)
   Research Article 6
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| DECEMBER | VOLUME 11 | N° 6 | 2020 |
| Info-AJIRAS-® Journal ISSN 2429-5396 (Online) / Reference  CIF/15/0289M |
  American Journal of Innovative Research & Applied Sciences
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*Correspondant author and authors Copyright © 2020:

| M’boh Epi Reine Elisabeth N'gou 1,2 | Chiaye Claire Antoinette Yapo-Crezoit 2 | Ama Valérie Bonouman 2 | Diane Kouao 2 | Toure offianan 2 | and | Allico Joseph Djaman 1,2 |

Affiliation.

1. Félix Houphouët Boigny University | Laboratory of biology and health |
2. Pasteur Institute of Côte d'Ivoire | Unity of Mycology | Unit of Immunology | Unit of biological Ressources |

This article is made freely available as part of this journal's Open Access: ID | M’boh–Ref3-ajira221120 |
Background: The cryopreservation for long period of mold strains of medical interest essential for research is complex. Our objective is to evaluate the impact of conservation with 10% of Glycerol and Brain Heart Infusion (BHI Broth) on the survival of molds of medical interest at -20°C. Methodology: This is an analytical study of the cultural and morphological characteristics of 1310 mold strains isolated from environmental samples (air, surface and dust) collected from all azimuths and preserved in 10% Glycerol and BHI Broth at -20°C for one year. Results:  Among the 1310 isolated strains and revived, colony growth was observed in 1059 strains (80.84%) against 251 (19.16%) which showed no signs of development and 119 strains (9.08%) were contaminated by other mold species or yeasts. Fungal viable strains observed after cryopreservation are: Aspergillus fumigatus, Aspergillus flavus, Aspergillus versicolor, Aspergillus niger, Aspergillus terreus, Aspergillus ochraceus, Aspergillus clavatus, Aspergillus sp, Penicillium sp, Fusarium solani, Fusarium sp, Curvularia sp, Mucor sp, Rhizomucor sp, and Trichoderma sp.  Conclusion: The ability to resist freezing in suspension medium 10% Glycerol and BHI Broth varied from strain to strain, but with a better cryopreservation for Aspergillus, Penicillium, Fusarium, Mucor, Rhizomucor and Trichoderma.
Keywords: Characterization, Mould, Environnement, Health, cryopreservation, Glycerol, BHI Broth
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American Journal of innovative
Research & Applied Sciences 
ISSN  2429-5396 (Online)
OCLC Number: 920041286
CHARACTERIZATION OF INDOOR MOLDS WITH ALLERGENIC POTENTIAL AFTER CRYOPRESERVATION AT -20°C FOR ONE YEAR

| M’boh Epi Reine Elisabeth N'gou 1,2 | Chiaye Claire Antoinette Yapo-Crezoit 2 | Ama Valérie Bonouman 2 | Diane Kouao 2 | Toure offianan 2 | and | Allico Joseph Djaman 1,2 |. Am. J. innov. res. appl. sci. 2020; 11(6):175-181.

  | PDF FULL TEXT | | XML FILE |   | Received | November 02, 2020 | | Accepted |  December 07, 2020 |  | Published | December 15, 2020 |


*Corresponding author Author & Copyright Author © 2020: | M’boh Epi Reine Elisabeth N'gou |. All Rights Reserved. All articles published in American Journal of Innovative Research and Applied Sciences are the  
property of Atlantic Center Research Sciences, and is protected by copyright laws CC-BY. See: http://creativecommons.org/licenses/by-nc/4.0/