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271
|Nina Nindum Sulem-Yong
1,2*
| Armand Fiemapong Nzoko
2
|Sophie Nina Natacha Eyenga | Ngono
1
| Patricia Linda
Kameni Djikengoue
1,2
| Serge Hubert Zebaze Togouet
2
| George Yongbi Chiambeng
1
| Pauline Mounjouenpou
1
|
Kingsley Agbor Etchu
1
| Steve Yong-Sulem
1
|
1.
Institute of Agricultural Research for Development | Yaoundé | Cameroon |
2.
University of Yaoundé I | Department of Animal Biology and Physiology | Yaoundé | Cameroon|
| Received | 20 Mai 2018 | | Accepted | 20 June 2018 | | Published 25 June 2018 |
ABSTRACT
Background: The main factor constraining Catfish farming consists of a chronic lack of fingerlings which is mostly due to very low
larval survival rates. Objective: In order to alleviate mass mortalities of larvae, zooplanktons were used as preys for
Clarias gariepinus
larvae. Methods: A completely randomized design was set up to test the effects of separate feeding with copepods, cladocerans,
rotifers and a 1:1:1 mixture of the three types for 20 days. Ingestibility, survival and growth were evaluated for pre-defined spanning
life stages labelled 1, 2, 3, 4 and 5 of four days each. Results: Rotifers were more ingested by stage 1 and 2 larvae than cladocerans
but stage 3 and 4 larvae ingested more of cladocerans. Larval survivals (100%) were not affected by zooplankton types during stage 1
but were significantly sustained by cladocerans during stage 2, 3, 4 and 5. Only in stage 3 did another zooplankton type, rotifers, stand
out as second in sustention of survivals. Larval growth was significantly affected right from stage 1, rotifers, cladocerans and mixtures
successively taking turns in excelling each other. Although copepods never excelled, they became increasingly important towards the
end of the experiment. Conclusions: These results were discussed to enable a conception of recommendations in view of optimizing
protocols for applying zooplanktons as preys for
C. gariepinus
larvae.
Keywords: Clarias gariepinus larvae, Zooplankton, Ingestibility, Survival, Growth.
1. INTRODUCTION
Clarias gariepinus
is the world’s biologically best aquaculture species [1]. It can accept and thrive on cheap feeds, it has a
high growth rate, it can tolerate high densities under culture conditions, it can resist most diseases, it is highly adapted to
tropical climates and it fetches high market prices in most sub Saharan African countries [2-4]. Its culture can therefore
constitute a basis for enhancement of food security and alleviation of malnutrition and poverty.
However, despite of the breakthrough reported for its artificial propagation [5,6], the demand for fish seed still outstrips
the supply [7]. The main factor constraining its culture in Cameroon consists of a chronic lack of fingerlings. This is
mostly due to a shortage of larval food which should not only contain the required nutrients but also enzymes for
digesting it [8] given that at this early stage, they lack awell-developed digestive system [9]. The brine shrimp,
Artemia
spp
.
contains both and is thus widely used for rearing commercially important freshwater and marine fish larvae
[10,11,12]. However, its high cost (45 USD/kg in Cameroon) and inaccessibility especially in rural zones [13,14] coupled
with the fact that being a marine species, it dies in freshwater within two hours due to osmoregulation [15,16] has
resulted in the search of financially accessible and suitable freshwater alternatives. Zooplanktons have been viewed as
potential alternatives for Artemia as live starter feed for
C. gariepinus
larvae [17,18,19] because of their excellent
morphological, behavioural and nutritional characteristics [20]. Theuse of mixed zooplankton [14-21] and specific species
of the rotifer group like
Brachionus calyciflorus
[18-20] and
Moina sp
of the Cladoceran group [21,22]have been used as
live starter feed for
C. gariepinus
larvae. However, there is paucity of information of the collective effect of species of
each of the main zooplankton groups on the survival and growth of
C. gariepinus
larvae.
The objective of this work was to enable alleviation of the mortalities and thus boost availability of fingerlings through
testing the effect of separate feeding with each of the zooplankton groups.
ORIGINAL ARTICLE
TOWARDS AN OPTIMUM PROTOCOL FOR FEEDING OF AFRICAN
CATFISH,
Clarias gariepinus
(BURCHELL, 1822), LARVAE WITH
DIFFERENT TYPES OF ZOOPLANKTONS PER LIFE STAGE
*Corresponding Author and Author Copyright © 2018: Nina Nindum Sulem-Yong. All Rights Reserved. All articles published in American Journal of Innovative
Research and Applied Sciences are the property of Atlantic Center Research Sciences, and is protected by copyright laws CC-BY. See: http://creativecommons.org/licenses/by-nc/4.0/.
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272
2. MATERIALS AND METHODS
2.1 Study area
The study was carried out at the aquaculture complex (Pure and Applied Zoology Laboratory) of the Department of
Animal Biology and Physiology of the University of Yaoundé I, Cameroon.
2.2 Production of African catfish larvae
Gravid brooders used for the experiment were obtained from a local fish farm in Nkoabang, Yaoundé, Cameroon. Larvae
of
Clarias gariepinus
were obtained through a method of artificial reproduction described by de Graaf and Janssen (1996)
[23].
2.3 Collection, identification and isolation of zooplanktons
Experimental zooplanktons were captured by towing a plankton net (mesh size of 64μm) through a eutrophic lake. A
representative sample of the zooplanktons was analysed right down to the species level as described by Braoini et al.,
(1983), Chiambeng (2004), Dumont and Negrea(2002), Dussart (1980), Dussart and Defaye (1995), Fernando (2000),
Smirnov and Timms (1983), Zebaze (2000) [24,25,26,27,28,29,30,31]. Identified copepods comprised Cyclopoids
(
Afrocyclops gibsoni
,
Mesocyclops salinus
*, Microcyclops sp.,
Tropocyclops confinis
) and copepodite nauplii. Cladocerans
comprised Chydorids, Moinids, Daphnids, Macrothricids (Table 1) and rotifers comprised Philodinids, Asplanchnids,
Brachionids, Colurellids, Lecanes, Mytilinids, Notommatids, Trichocercids,Bdelloids (Table 2).
Table 1: The table presents the species composition of Cladocerans.
Chydoridae
Daphnidae
Macrothricidae
Moinidae
Chydorus eurynotus*
Ceriodaphnia cornuta
Guernella raphaelis
Moina micrura*
C. globules*
Macrothrix spinosa
Kurzial ongirostris
M. laticornis
K. latissima
Pleuroxus denticulatus
Alona guttata
*: Most ingested species
Table 2:The table presents the species composition of rotifers.
Philodinidae
Asplanchnidae
Brachionidae
Colurellidae
Notommatidae
Trichocercidae
Rotaria
citrine*
Asplanchna
brightwelli*
Anureopsis fissa
Mytilina mitica
Cephalodella gibba
Trichocerca
bicristata
Rotaria sp.
A. priodonta
Brachionus angularis*
M. mucronata
C. bottgeri
T. chattoni
B. calyciflorus*
Notommata codonella
T. stillata
B. falcatus*
N.pseudocerberus
T. tchadiensis
B. quadridentatus*
Macrochaetus sp.
Trichotria
tetractis
B. leydigi
Keratella quadrata
Platyias leloupi
P. quadriconnis
*: Most ingested species.
The collected water samples were filtered using 64μm mesh size plankton net and rotifers were retained. The samples
were later filtered using a 100μm mesh size net to collect copepods and cladocerans. In order to reduce contamination
by unwanted organisms, the various zooplankton group samples were thoroughly rinsed with distilled water.
2.4 Experimental setup
The experimental set-up consisted of a completely randomized design for testing effects of copepods (Treatment 1),
cladocerans (Treatment 2), rotifers (Treatment 3) and a 1:1:1 mixture of the three zooplankton groups (Treatment 4), on
average ingestibility, survival and growth spanning life stages (5) of four days each of
Clarias gariepinus
larvae. It should
be noted that the growth spanning ages defined (1, 2 3, 4 and 5 of every four days each) in the present study was to be
able to ease the presentation of results and explanations.
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273
Initial average weight (3mg) of experimental larvae was determined by measuring the parameters of a random sample of
30 three-day old larvae which had just completely absorbed their yolk sacs and were ready for exogenous feeding.
Weighing was done by using a sensitive electronic balance (Sartorius, ±1mg).
Prior to experimentation, nursing water (3liters) contained in each of twelve plastic basins was exposed to air for 24hours
to favour vaporization of chlorine usually used to sterilize the water. Each basin was then stocked with fifty larvae
corresponding to a density of almost17larvae/litre. Thereafter, three of the basins, chosen at random, were assigned to
each of the four treatments. Throughout the experiment (20 days), water temperature, pH, dissolved oxygen, and total
dissolved solids (TDS) as monitored every four days, remained acceptable for larval rearing [20-32, 33].Water
temperature varied from 24.8 to 25°C, pH revolved around neutrality (7CU), dissolved oxygen ranged from 5.18 to
7.06mg/l while total dissolved solids ranged from 51.33 to 63.1mg/l.
For feeding of larvae, the zooplanktons were captured every other day and separated into copepods, cladocerans and
rotifers, using a micropipette and a pair of binoculars (WILD M5). Thereafter, 10ml of separated concentrate, respectively
averaging 362 copepods, 3134 cladocerans and 2816 rotifers; were collected with a syringe and thoroughly washed with
tap water prior to feeding [14] larvae of Treatments 1, 2 and 3 respectively. For feeding Treatment 4 with mixed
zooplanktons, 3.3ml from each of the separate concentrates, containing a total of about 2018 zooplanktons, was used. All
larvae were fed thrice a day (07H00, 15H00, and 18H 00). Morning rations were applied after cleaning of holding basins
and partial (about 66%) replacement of holding water with fresh one. Cleaning and reduction of water were done by
siphoning with a rubber tube (2mm).
2.5 Data collection and analysis
For determination of ingestibility, the number of zooplanktons remaining in the holding basins per day was estimated by
counting the number contained in homogenized samples (10ml) of holding water. Ingestibility was defined, within the
context of this work, as the ratio of the difference between the zooplankton number that was injected the previous day
(ni) and the number that was found the following day (nf) to the number that was injected (ni):
Ingestibility = ((ni-nf)/ni)x 100 (1)
It should be noted that copepods were difficult to capture due to their dodgy character, hence their leftovers were only
estimated on the last day of the experiment. Therefore, it was not possible to determine ingestibilities for treatments 1
and 4- it was only done for treatments 2 and 3. Dead larvae removed on a daily basis were counted to enable calculation
of daily survivals per treatment. Overall, survivals were calculated after counting all the larvae (nf) remaining on day 20
according to the following formula:
Percentage survival= (Nf/50) x100 (2)
As for growth performance, 10 larvae were randomly sampled every four days, from each basin and their weights at the
beginnings and ends of each stage (Wt
1
and Wt
2
) were measured using an electronic balance (Sartorius, ±1mg). Specific
growth rates (SGR) were then calculated according to the following formula:
SGR = ((Ln Wt
2
– LnWt
1
) x 100)/20 (3)
Ingestion, survival and growth parameters of the various treatments were subjected to ANOVA using SPSS (version
17.0). Differences were considered significant at p<0.05.
3. RESULTS
The ingestibilities of rotifers and cladocerans by
Clarias gariepinus
larvae per stage are shown in Table 3. Rotifer
ingestibilities were negatively correlated (correlation factor = -0.49) with the age of larvae while those of cladocerans
were positively so (correlation factor=+0.12). The lowness of the correlation factors can be explained by the facts that
the decreasing trend of rotifer ingestibility was reversed at a larval age of 8-12 days (growth spanning ages 1,2 and 3)
while the increasing trend of cladocerans ingestibility was reversed at a larval age of 13-23 days (growth spanning ages 4
and 5).
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274
Table 3: The table presents the ingestibilities of cladocerans and rotifers
by
C. gariepinus
larvae per four-day period.
Larval Age
Rotifers
Cladocerans
4 - 8 days
83.5
72.77
9 - 13 days
76.75
75.21
14 - 17 days
86.06
95.84
18 - 21 days
78.03
81.42
22 - 25 days
75.94
72.12
The effect of feeding with copepods, cladocerans, rotifers and a 1:1:1 mixture of the three zooplankton groups on the
survival of
C. gariepinus
larvae is shown in Figure 1.
Figure 1: The figure presents the effect of feeding with copepods, cladocerans,
rotifers and mixed zooplanktons on survival (%) of
C. gariepinus
larvae per period of
four days.
Irrespective of treatments, survival remained 100% for larvae of upto9 days old (growth spanning ages 1 and 2) and
dropped gradually for those of 10 and 11 days old (growth spanning age 3), with the sharpest drops being registered at
an age of 12 days old. Not until13 days did treatments begin to affect larval survival? Cladocerans-fed larvae suffered the
least mortality while copepod-fed ones suffered the greatest. This trend of significantly higher survival (p<0.05) of
cladoceran-fed larvae was maintained right up to an age of 23 days (growth spanning age 5). Ingestion of cladocerans
thus significantly sustained the survival of larvae. With respect to survival sustention, rotifers seconded cladocerans
during stage 3. Irrespective of the stage, there were no significant differences (p>0.05) in the survival-sustention effects
of copepods and mixtures.
The effect of the treatments on the growth of
C. gariepinus
larvae per stage is presented in figures 2 and 3. Rotifers
conferred a significantly higher (p<0.05) SGR to larvae of 3 to 7 days old than all the other treatments. Cladoceran-
conferred SGRs were significantly higher (p<0.05) for larvae of 8 to 16 (growth spanning ages 2, 3 and 4) days old, a
rank which was taken over by mixture conferred SGRs for larvae of 16 to 23 days old(growth spanning ages 4 and 5). In
general, rotifers, cladocerans and mixtures successively took turns in excelling each other. They were respectively
seconded by mixtures, copepods and cladocerans. Over the 20-day experimental period, attained final weight and specific
growth rate decreased as diet changed from mixed zooplankton through cladocerans and copepods to rotifers.
0
2
4
6
8
10
12
14
16
0 4 8 12 16 20
Weight (mg)
Days
Copepods Cladocerans Rotifers Mixed Zooplankton
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Figure 2: The figure presents the effect of feeding with copepods, cladocerans,
rotifers and mixed zooplanktons on weight (mg) gain by
C. gariepinus
larvae per
four-day period.
Figure 3: The figure presents the effect of copepods, cladocerans, rotifers and mixed
zooplankton on the evolution of specific growth rates (%) of
C. gariepinus
larvae with time.
4. DISCUSSION
Reversals of rotifers and cladocerans ingestibilities with respect to growth spanning ages are believed due to larvae’s
non-selective filter feeding habit and to the capacity of more developed larvae to also ingest copepods. It would appear
that
Clarias gariepinus
larvae kept ingesting more cladocerans than rotifers until they were also able to prey on the dodgy
copepods. This preference for larger preys in spite of their high swimming activity could be attributed to the high
swimming activity and predation of the fish larvae as well [34,35].
Results demonstrated for the first time that the survival and growth of zooplankton-fed
C. gariepinus
larvae can only be
optimized if the kinds of the zooplanktons are tailored to specific life stages of larvae. While survival of up to two-week-
old larvae seemed to be more dependent on extra experimental factors, those of more than two-week-old portrayed
cladocerans as the best feed. This was corroborated in terms of growth as it excelled the other diets for larvae of 7 to 15
days old and remained competitive for larvae of 16 to23 days old. However, it should be noted that rotifers excelled for
larvae of 3 to 6 days while mixtures excelled in those of 16 to 23 days. That the suitability of rotifers for 3 to 6 days old
larvae was closely seconded only by the mix diet in which they were also present underscores their importance as preys
for this stage of larvae. Such larvae should therefore be fed with pure rotifers or with diets in which they are abundantly
represented. This was in conformity with the works of Agadjihouèdéet al., (2012)[14] who used a mixture of the three
zooplankton groups to feed
C. gariepinus
larvae with rotifers representing the highest percentage (78%).
That rotifers stood out as the best zooplankton preys at the onset of exogenous feeding confirms the findings of Arimoro,
Awaïss et al., (1993) and Wang et al., (2005) who observed that freshwater rotifers can be successfully used as starter
feed for
C. gariepinus
larvae [20,36,37]. This can also be attributed to the match between their small sizes and the small
mouth gapes of the larvae. It would appear that whenever gapes can permit, larvae will go for bigger than for smaller
preys [38], even abstaining from smaller preys at the risk of starving as indicated by the negative correlation between
ingestion of rotifers and age of larvae here observed.
0
2
4
6
8
10
12
14
16
0 4 8 12 16 20
Weight (mg)
Days
Copepods Cladocerans Rotifers Mixed Zooplankton
0
5
10
15
20
25
4 8 12 16 20
Specific growth rates
Days
Copepods Cladocerans Rotifers Mixture
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That cladocerans were not so consumed at the beginning and that they took over as the best preys for larvae of 7 to 14
days old could have resulted from the match between their sizes and mouth gapes of larvae. Therefore, larvae of 7 to 14
days old should be fed with cladocerans. These findings are similar to the works of Adejoke (2015) who reported that
C.
gariepinus
larvae fed with cladocerans exhibited good growth performance and survival [39].
The confirmation of the decisiveness of the match between prey size and mouth gape came from the fact that copepods
conferred higher and higher growth rates as the larvae grew older and older (16 to 23 days old). This growth
performance of copepod-fed larvae could be due to the better nutritional value (higher essential fatty acids) when
compared to the other live feeds [40]. The relative performance of copepods and cladocerans during this period suggests
that it is mostly thanks to the two that the mix diet performed best. The African catfish
C. gariepinus
larvae of 16 to 23
days old should therefore prefer larger preys like cladocerans and copepods. This preference for larger size preys has
also been observed in
Heterobranchus longifilis
larvae, in juvenile Pollock (
Theragrachalco gramma
) and in larval yellow
perch by Ajah (2010), Brodeur (1998) and Graeb et al., (2004) respectively [41,42-38]. The question why predators
should prefer larger preys to many small one amounting to the same weight begs for further research. Growth
performance obtained for mixed zooplankton- fed larvae was more important than those reported by Awaïss et al.,
(1993) and Agadjihouèdé et al., (2012) [36-14].
The mass mortalities of week-old
C. gariepinus
larvae reported by Yong-Sulem (2011) recurred in this experiment
regardless of the treatment [21]. Had it been due to clogging of gills or physical attack suspected by Yong-Sulem (2011),
treatment 3 with only rotifers which neither clog nor attack would not have suffered them [21]. Even treatment 2 with
only cladocerans would have been spared. Only the large sized copepods have been reported to constitute a nuisance of
any sort to larvae: -Schäperclaus (1992) reported that fish larvae are generally attacked by adult copepods and advanced
copepodite stages resulting in serious lesions of fins, head, nares and particularly the gills [43]. Therefore, the hypothesis
of Yong-Sulem (2011) does not apply to all groups of preys [21]. The mortalities observed by Yong-Sulem (2011) and in
the present experiment are more likely to do with lack of nutrients by certain species of zooplanktons from certain
biotopes [21]. This is corroborated by the fact that zooplanktons from a similar source (hyper-eutrophic reservoir) equally
sustained low survival of
C. gariepinus
larvae, 31% as early as 10 days old [34]. According to Agadjihouèdé et al.,
(2012), good survival and growth performance of
C. gariepinus
larvae fed with freshwater zooplankton results from the
excellent digestibility and good nutrient quality of the prey [14].
Ajah (2010) proved that the gustatory and chemoreceptor organs of
C. gariepinus
larvae were developed during the
second week of life and it is thought that such development requires more nutrients than were present in those of un-
enriched zooplanktons [41]. To avoid survival crashes during the period of organogenesis, prey zooplanktons should be
enriched. Such enrichment is known to more than double survival of African catfish
C. gariepinus
[20] and thought to be
able to optimize both survival and growth if compounded with tailoring to specific life stages.
5. CONCLUSION
These results demonstrated that the survival and growth of zooplankton-fed
Clarias gariepinus
larvae can only be
optimized if the kinds of the zooplanktons are tailored to specific life stages of larvae. Rotifers were the best preys of 3 to
6 day old larvae, followed by cladocerans of 7 to 14 day old larvae and as they grow older a mixture of cladocerans and
copepods of 16 to 23 day old larvae should be given as proven by their high growth and survival performances. The low
survivals observed during this experiment could be attributed to the poor nutrient quality of the preys. In order to avoid
such survival crashes, preyed zooplankton should be enriched as such enrichment has shown to double survival of the
African catfish
C. gariepinus
. Experiments are required to optimize enrichment regimes for desired intends and purposes.
Acknowledgements
The authors are grateful to Dr Kekeunou Sévilor of the Zoology and Parasitology laboratory of the University of Yaoundé I
for providing the infrastructure facilities used for this research. The Institute of Agricultural Research for Development
(IRAD) and the International Institute of Tropical Agriculture (IITA) are highly appreciated for offering unlimited access to
their laboratories.
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Citer cet article: Nina Nindum Sulem-Yong, Armand F. Nzoko, Sophie Nina Natacha Eyenga Ngono, Patricia Linda Kameni
Djikengoue, Serge Hubert Zebaze Togouet, George Yongbi Chiambeng, Pauline Mounjouenpou, Kingsley Agbor Etchu, and
Steve Yong-Sulem. TOWARDS AN OPTIMUM PROTOCOL FOR FEEDING OF AFRICAN CATFISH, Clarias gariepinus (BURCHELL,
1822), LARVAE WITH DIFFERENT TYPES OF ZOOPLANKTONS PER LIFE STAGE. American Journal of Innovative Research and Applied
Sciences. 2018; 6(6): 270-278.
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